5NC1
Structure of the distal domain of mouse adenovirus 2 fibre bound to N-acetyl-glucosamine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-31 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.8729 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 163.410, 163.410, 163.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.000 - 2.000 |
| R-factor | 0.16736 |
| Rwork | 0.165 |
| R-free | 0.20513 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5nbh |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.444 |
| Data scaling software | Aimless |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.900 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.101 | 0.404 |
| Number of reflections | 48930 | 3642 |
| <I/σ(I)> | 13.2 | 4.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.9 | 7.8 |
| CC(1/2) | 0.998 | 0.714 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 1.125 M lithium sulphate, 0.075 M HEPES-NaOH, 25% (v/v) glycerol, 10 mM Tris-HCl |
