5NBT

Apo structure of p60N/p80C katanin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-13
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.000020
Spacegroup name	C 1 2 1
Unit cell lengths	146.140, 37.760, 103.090
Unit cell angles	90.00, 93.37, 90.00

Refinement procedure

Resolution	43.262 - 2.400
R-factor	0.2469
Rwork	0.244
R-free	0.29400
Structure solution method	MAD
RMSD bond length	0.003
RMSD bond angle	0.582
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.262	2.600
High resolution limit [Å]	2.400	2.400
Rmerge	0.165	1.404
Number of reflections	22520	4734
<I/σ(I)>	8.9	1.46
Completeness [%]	99.9	100
Redundancy	6.7	7
CC(1/2)	0.998	0.659

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	293	27% PEG 3350, 0.1 M BisTris propane (6.0), 0.2 M MgCl2