5NBK

NDM-1 metallo-beta-lactamase: a parsimonious interpretation of the diffraction data

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-09-13
Detector	DECTRIS PILATUS 2M-F
Spacegroup name	P 41 21 2
Unit cell lengths	108.047, 108.047, 92.571
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.660 - 2.600
R-factor	0.18298
Rwork	0.181
R-free	0.22928
RMSD bond length	0.013
RMSD bond angle	1.504
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall
Low resolution limit [Å]	46.660
High resolution limit [Å]	2.600
Number of reflections	17411
<I/σ(I)>	999
Completeness [%]	100.0
Redundancy	9.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	24 % PEG 1500 AND 0.05 M TBG (SODIUM TARTRATE/BIS - TRIS/GLYCYLGLYCINE) PH 4