5NBH
Structure of the distal domain of mouse adenovirus 2 fibre, P212121 native
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9763 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.872, 91.110, 128.922 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.050 - 1.700 |
R-factor | 0.17422 |
Rwork | 0.172 |
R-free | 0.21621 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5n8d |
RMSD bond length | 0.012 |
RMSD bond angle | 1.509 |
Data scaling software | Aimless |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.100 | 1.740 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.116 | 0.233 |
Number of reflections | 68912 | 3722 |
<I/σ(I)> | 6.3 | 2.1 |
Completeness [%] | 94.3 | 69.6 |
Redundancy | 3.5 | 1.9 |
CC(1/2) | 0.983 | 0.857 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 10 mM Tris-HCl, 10 % (w/v) PEG 4000, 0.1 M HEPES-NaOH, 0.1 M magnesium chloride |