5NBC
Structure of Prokaryotic Transcription Factors
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-09-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97, 1.77 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.150, 90.000, 63.950 |
| Unit cell angles | 90.00, 93.48, 90.00 |
Refinement procedure
| Resolution | 42.076 - 1.699 |
| R-factor | 0.2167 |
| Rwork | 0.215 |
| R-free | 0.24390 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.874 |
| Data scaling software | XSCALE (VERSION November 3, 2014 BUILT=20141118) |
| Phasing software | SHELXCD (VERSION 2014/2) |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 42.080 |
| High resolution limit [Å] | 1.690 |
| Number of reflections | 64620 |
| <I/σ(I)> | 11.66 |
| Completeness [%] | 97.7 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.05M MES pH 5.8, 20% W/V PEG 3350 0.2 M Magnesium chlorides hexahydrate 0,01mM of MnCl2 |
