5NB0

Crystal structures of homooligomers of collagen type IV. alpha3NC1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-26
Detector	RAYONIX MX-225
Wavelength(s)	1.0
Spacegroup name	H 3
Unit cell lengths	131.518, 131.518, 248.855
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	51.780 - 2.700
R-factor	0.1639
Rwork	0.163
R-free	0.17580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1t60
RMSD bond length	0.008
RMSD bond angle	1.146
Data reduction software	XDS
Data scaling software	SCALA (3.3.16)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	51.783	8.540	2.850
High resolution limit [Å]	2.700	6.040	2.700
Rmerge		0.034	0.264
Rmeas	0.110	0.044	0.347
Rpim	0.069	0.027	0.223
Total number of observations	96546
Number of reflections	43361
<I/σ(I)>	11.2	17.3	2.1
Completeness [%]	98.4	99.4	95
Redundancy	2.2	2.5	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	6% PEG3350, BisTrisPropane pH 7.5 and MgCl2 0.2 M