5NAX
Crystal structures of homooligomers of the non-collagenous domains of collagen type IV. alpha121NC1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-11-05 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.25 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 126.148, 126.148, 216.211 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.450 - 2.820 |
| R-factor | 0.1431 |
| Rwork | 0.141 |
| R-free | 0.18480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1t60 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.187 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.750 | 48.447 | 2.970 |
| High resolution limit [Å] | 2.820 | 8.920 | 2.820 |
| Rmerge | 0.054 | 0.367 | |
| Rmeas | 0.101 | 0.060 | 0.405 |
| Rpim | 0.040 | 0.026 | 0.166 |
| Total number of observations | 258124 | 7974 | 36709 |
| Number of reflections | 48121 | ||
| <I/σ(I)> | 12.7 | 23.4 | 3.6 |
| Completeness [%] | 98.9 | 95.8 | 99.8 |
| Redundancy | 5.4 | 4.9 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 10% PEG8000, Magnesium acetate 0.2 M |
