5NAV
Crystal structure of the double mutant (Cys211Ser/Cys292Ser) 6-phospho-b-D-glucosidase from Lactobacillus plantarum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-05-16 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.8726 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 196.740, 192.060, 105.840 |
Unit cell angles | 90.00, 102.39, 90.00 |
Refinement procedure
Resolution | 38.380 - 2.300 |
R-factor | 0.1648 |
Rwork | 0.163 |
R-free | 0.20570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5naq |
RMSD bond length | 0.008 |
RMSD bond angle | 0.979 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.420 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.113 | 0.569 |
Rpim | 0.067 | 0.353 |
Number of reflections | 169552 | 24703 |
<I/σ(I)> | 8.7 | |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 3.8 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 20% (w/v) PEG 3350, 0.2 M sodium citrate and 50 mM spermidine |