5NAA
Lipoprotein-releasing system transmembrane protein LolC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-01-16 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 54.680, 111.780, 75.939 |
| Unit cell angles | 90.00, 108.97, 90.00 |
Refinement procedure
| Resolution | 45.990 - 1.880 |
| R-factor | 0.18223 |
| Rwork | 0.180 |
| R-free | 0.22520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5lil |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.806 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.990 | 1.920 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.078 | 0.436 |
| Number of reflections | 34822 | |
| <I/σ(I)> | 11 | |
| Completeness [%] | 99.5 | |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 288.15 | 0.2 M Ammonium sulfate, 0.1 M Sodium acetate pH 4.6, 30% w/v PEG 2000 MME |
