5NA2
Crystal structure of the full-length Feline Immunodeficiency Virus capsid protein unveils original features
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-10 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.99187 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 122.190, 74.580, 77.000 |
| Unit cell angles | 90.00, 128.68, 90.00 |
Refinement procedure
| Resolution | 38.499 - 1.670 |
| R-factor | 0.1992 |
| Rwork | 0.197 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2XGU (N-terminal domain) and 5DCK (C-terminal domain) |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.888 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.719 | 2.010 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmeas | 0.033 | |
| Number of reflections | 39411 | |
| <I/σ(I)> | 16.42 | 3.33 |
| Completeness [%] | 91.2 | 93 |
| Redundancy | 2.38 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 0.2 M magnesium sulfate, 20% PEG 4000, 10% glycerol, 10% DMSO |
