5N9Z
Rubisco from Thalassiosira hyalina
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 117.992, 219.986, 124.274 |
| Unit cell angles | 90.00, 118.34, 90.00 |
Refinement procedure
| Resolution | 62.029 - 1.899 |
| R-factor | 0.1521 |
| Rwork | 0.150 |
| R-free | 0.18220 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.973 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 97.900 | 1.970 |
| High resolution limit [Å] | 1.899 | 1.900 |
| Rmerge | 0.066 | 0.251 |
| Number of reflections | 432731 | 42903 |
| <I/σ(I)> | 5 | |
| Completeness [%] | 99.2 | 98.5 |
| Redundancy | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 26% PRG4000, 0.1M HEPES pH 8.5, 0.1M sodium chloride, 0.005M magnesium chloride, 0.01M sodium hydrogen carbonate |
