5N9O
EGFP(enhanced green fluorescent protein) mutant - L232H
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.972 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 84.595, 67.931, 47.266 |
Unit cell angles | 90.00, 118.66, 90.00 |
Refinement procedure
Resolution | 12.500 - 1.530 |
R-factor | 0.16948 |
Rwork | 0.167 |
R-free | 0.21146 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4l12 |
RMSD bond length | 0.021 |
RMSD bond angle | 2.509 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.480 | 1.580 |
High resolution limit [Å] | 1.530 | 1.530 |
Rmerge | 0.073 | 0.510 |
Rpim | 0.048 | 0.380 |
Number of reflections | 34978 | 3431 |
<I/σ(I)> | 8.6 | 1.8 |
Completeness [%] | 98.8 | 99.5 |
Redundancy | 3.3 | 3.3 |
CC(1/2) | 0.589 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 288 | 2-propanol , MES monohydrate, PEG 2000 |