5N99
CRYSTAL STRUCTURE OF STREPTAVIDIN with cyclic peptide NQpWQ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-27 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.99999 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.744, 115.315, 210.369 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.720 - 1.500 |
| R-factor | 0.1684 |
| Rwork | 0.167 |
| R-free | 0.19170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.6) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.720 | 1.600 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 261661 | 44516 |
| <I/σ(I)> | 13.3 | 1.49 |
| Completeness [%] | 99.7 | 98.4 |
| Redundancy | 6.58 | 5.77 |
| CC(1/2) | 0.999 | 0.447 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1M Sodium citrate pH 5.5 15% PEG6000 |
