5N98

Crystal Structure of Drosophila DHX36 helicase in complex with TAGGGTTTT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9791
Spacegroup name	C 1 2 1
Unit cell lengths	303.408, 51.615, 164.965
Unit cell angles	90.00, 114.43, 90.00

Refinement procedure

Resolution	56.840 - 2.756
R-factor	0.1778
Rwork	0.175
R-free	0.23650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5n8r
RMSD bond length	0.011
RMSD bond angle	1.578
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((dev_2427: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.840	2.855
High resolution limit [Å]	2.756	2.756
Rmerge	0.112	0.680
Number of reflections	56626	6072
<I/σ(I)>	10.28	2.16
Completeness [%]	92.5	99.87
Redundancy	3.7	3.8
CC(1/2)	0.994	0.721

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	0.1M MES monohydrate-imidazole 20mM sodium formate 20mM ammonium acetate 20mM sodium citrate tribasic hydrate 20mM sodium oxamate 20mM potassium sodium tartrate tetrahydrat