5N94
Crystal Structure of Drosophila DHX36 helicase in complex with polyU
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9799 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 338.328, 51.534, 159.899 |
| Unit cell angles | 90.00, 118.15, 90.00 |
Refinement procedure
| Resolution | 46.997 - 2.428 |
| R-factor | 0.215 |
| Rwork | 0.212 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5n8r |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.454 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.000 | 2.515 |
| High resolution limit [Å] | 2.428 | 2.428 |
| Rmerge | 0.047 | 0.531 |
| Number of reflections | 84548 | 9161 |
| <I/σ(I)> | 17.44 | 2.46 |
| Completeness [%] | 90.7 | 99.41 |
| Redundancy | 3.3 | 3.5 |
| CC(1/2) | 0.999 | 0.878 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.1M MES monohydrate-imidazole 20mM sodium formate 20mM ammonium acetate 20mM sodium citrate tribasic hydrate 20mM sodium oxamate 20mM potassium sodium tartrate tetrahydrate |
