5N8Z

Crystal Structure of Drosophila DHX36 helicase in complex with CTCTCCCTT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-05-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9791
Spacegroup name	C 1 2 1
Unit cell lengths	306.251, 51.171, 165.177
Unit cell angles	90.00, 115.30, 90.00

Refinement procedure

Resolution	40.000 - 3.477
R-factor	0.2011
Rwork	0.198
R-free	0.27350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5n8r
RMSD bond length	0.012
RMSD bond angle	1.415
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.980	3.601
High resolution limit [Å]	3.470	3.477
Rmerge	0.169	0.644
Number of reflections	29426	3035
<I/σ(I)>	9.06	2.36
Completeness [%]	95.9	99.67
Redundancy	3.7	3.8
CC(1/2)	0.989	0.660

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	0.1M MES monohydrate-imidazole 20mM sodium formate 20mM ammonium acetate 20mM sodium citrate tribasic hydrate 20mM sodium oxamate 20mM potassium sodium tartrate tetrahydrate