5N8T

CRYSTAL STRUCTURE OF STREPTAVIDIN D-amino acid containing peptide Gdlwqheatwkkq

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-16
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.700001
Spacegroup name	P 42 21 2
Unit cell lengths	57.779, 57.779, 84.405
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.720 - 1.610
R-factor	0.20587
Rwork	0.204
R-free	0.24341
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.526
Data reduction software	XDS
Data scaling software	SADABS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.720	1.710
High resolution limit [Å]	1.610	1.610
Rmerge		0.950
Number of reflections	18228	3098
<I/σ(I)>	14.94	0.86
Completeness [%]	100.0	100
Redundancy	12.48
CC(1/2)	0.990	0.341

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	293	0.1M Sodium citrate pH 4.5, 20% PEG4000