5N8R
Crystal Structure of Drosophilia DHX36 helicase in complex with GAGCACTGC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 300.866, 51.477, 164.393 |
| Unit cell angles | 90.00, 114.22, 90.00 |
Refinement procedure
| Resolution | 149.900 - 2.200 |
| R-factor | 0.1782 |
| Rwork | 0.176 |
| R-free | 0.21860 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.149 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | autoSHARP |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 149.900 | 2.279 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.073 | 0.608 |
| Number of reflections | 117031 | 11661 |
| <I/σ(I)> | 13.08 | 2.36 |
| Completeness [%] | 99.3 | 99.52 |
| Redundancy | 3.7 | 3.7 |
| CC(1/2) | 0.998 | 0.738 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.1M MES monohydrate-imidazole 20mM sodium formate 20mM ammonium acetate 20mM sodium citrate tribasic hydrate 20mM sodium oxamate 20mM potassium sodium tartrate tetrahydrate |
