5N8Q
Structure of truncated Norcoclaurine Synthase from Thalictrum flavum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-10-02 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.970 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 38.046, 109.632, 136.739 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 85.680 - 2.000 |
R-factor | 0.199 |
Rwork | 0.197 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2VQ5 CHAIN A |
RMSD bond length | 0.022 |
RMSD bond angle | 2.078 |
Data reduction software | DIALS (1.0-0-g66ac5d4) |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 109.630 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.147 | 1.141 |
Number of reflections | 39359 | |
<I/σ(I)> | 6.1 | 1.7 |
Completeness [%] | 99.3 | 99.7 |
Redundancy | 5.4 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 10% w/v polyethylene glycol 1000, 10% w/v polyethylene glycol 8000 20 mM Tris, 50 mM NaCl, ph 7.5 |