5N8Q
Structure of truncated Norcoclaurine Synthase from Thalictrum flavum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-02 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.970 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 38.046, 109.632, 136.739 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 85.680 - 2.000 |
| R-factor | 0.199 |
| Rwork | 0.197 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2VQ5 CHAIN A |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.078 |
| Data reduction software | DIALS (1.0-0-g66ac5d4) |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 109.630 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.147 | 1.141 |
| Number of reflections | 39359 | |
| <I/σ(I)> | 6.1 | 1.7 |
| Completeness [%] | 99.3 | 99.7 |
| Redundancy | 5.4 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 10% w/v polyethylene glycol 1000, 10% w/v polyethylene glycol 8000 20 mM Tris, 50 mM NaCl, ph 7.5 |
