5N8E
CRYSTAL STRUCTURE OF STREPTAVIDIN WITH PEPTIDE RDPAPAWAHGGG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-01 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.99992 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.810, 84.733, 58.497 |
| Unit cell angles | 90.00, 99.40, 90.00 |
Refinement procedure
| Resolution | 57.780 - 1.100 |
| R-factor | 0.13551 |
| Rwork | 0.135 |
| R-free | 0.15291 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.332 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.800 | 1.200 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Number of reflections | 167056 | 37631 |
| <I/σ(I)> | 15.2 | |
| Completeness [%] | 96.7 | 90.5 |
| Redundancy | 3.17 | |
| CC(1/2) | 0.999 | 0.388 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293 | 0.2M Ammonium acetate 0.1M Sodium acetate pH 4 15% PEG4000 |
