5N8B
CRYSTAL STRUCTURE OF STREPTAVIDIN WITH PEPTIDE AFPDYLAEYHGG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-08-19 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.77491 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.319, 65.763, 78.133 |
| Unit cell angles | 90.00, 103.72, 90.00 |
Refinement procedure
| Resolution | 75.900 - 1.030 |
| R-factor | 0.13904 |
| Rwork | 0.138 |
| R-free | 0.15537 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.369 |
| Data reduction software | XDS (sad) |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.900 | 1.120 |
| High resolution limit [Å] | 1.030 | 1.030 |
| Rmerge | 0.045 | 0.650 |
| Number of reflections | 235380 | 54696 |
| <I/σ(I)> | 9.44 | |
| Completeness [%] | 99.5 | 99.7 |
| Redundancy | 3.38 | 3.33 |
| CC(1/2) | 0.999 | 0.367 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 0.1M Magnesium chloride 0.1M Sodium citrate pH 5 15% PEG4000 |
