5N89
CRYSTAL STRUCTURE OF STREPTAVIDIN WITH PEPTIDE GNSFDDWLASKG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-05 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.70000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 58.310, 58.305, 88.285 |
| Unit cell angles | 104.38, 91.03, 88.43 |
Refinement procedure
| Resolution | 85.510 - 1.270 |
| R-factor | 0.18141 |
| Rwork | 0.180 |
| R-free | 0.20373 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.415 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.510 | |
| High resolution limit [Å] | 1.270 | |
| Rmerge | 0.072 | |
| Number of reflections | 273857 | |
| <I/σ(I)> | 7.71 | |
| Completeness [%] | 97.0 | 95.7 |
| Redundancy | 1.74 | 1.7 |
| CC(1/2) | 0.998 | 0.378 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M Potassium chloride 0.1M HEPES pH 7.5 15% PEG6000 |
