5N85
Structure of RPA70N in complex with PrimPol (fragment 514-525)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-07 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.860, 53.090, 54.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.118 - 2.000 |
| R-factor | 0.1891 |
| Rwork | 0.187 |
| R-free | 0.22750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ipc |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.785 |
| Data reduction software | MOSFLM (7.1.0) |
| Data scaling software | SCALA (3.3.21) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 38.073 | 31.118 | 2.110 |
| High resolution limit [Å] | 2.000 | 6.320 | 2.000 |
| Rmerge | 0.069 | 0.751 | |
| Rmeas | 0.226 | 0.072 | 0.790 |
| Rpim | 0.062 | 0.021 | 0.239 |
| Total number of observations | 100895 | 3264 | 11524 |
| Number of reflections | 7856 | ||
| <I/σ(I)> | 10.9 | 25.4 | 3 |
| Completeness [%] | 99.6 | 99.4 | 98.1 |
| Redundancy | 12.8 | 11.1 | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293.15 | 0.2 M Ammonium acetate 0.1 M Sodium acetate 4.5 20 % w/v PEG 3350 |
