5N7I
Crystal structure of the coiled-coil domain of human tricellulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-13 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.918409 |
| Spacegroup name | P 42 |
| Unit cell lengths | 95.711, 95.711, 34.615 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.860 - 2.880 |
| R-factor | 0.212 |
| Rwork | 0.210 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5n7h |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.860 | 3.220 |
| High resolution limit [Å] | 2.879 | 2.880 |
| Rmerge | 0.079 | 1.332 |
| Number of reflections | 7271 | 711 |
| <I/σ(I)> | 14.62 | 1.33 |
| Completeness [%] | 98.4 | 98.78 |
| Redundancy | 5.4 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 8.5% (w/v) PEG4000, 20% 2-propanol, 0.1 M HEPES-NaOH pH 7.0 |
