5N7D
MAGI-1 complexed with a RSK1 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-07-06 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9762 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 195.810, 60.680, 99.710 |
Unit cell angles | 90.00, 98.81, 90.00 |
Refinement procedure
Resolution | 49.267 - 2.300 |
R-factor | 0.1923 |
Rwork | 0.191 |
R-free | 0.22710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xjl |
RMSD bond length | 0.003 |
RMSD bond angle | 0.569 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((dev_2420: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.267 | 2.360 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmeas | 0.773 | |
Number of reflections | 51873 | |
<I/σ(I)> | 18 | 2.36 |
Completeness [%] | 100.0 | 100 |
Redundancy | 12.8 | 8.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 296 | 8% PEG 400, 100 mM acetate buffer |