5N76

Crystal structure of the apo-form of the CO dehydrogenase accessory protein CooT from Rhodospirillum rubrum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Collection date	2016-02-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.987
Spacegroup name	P 41 21 2
Unit cell lengths	108.943, 108.943, 110.734
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.590 - 1.900
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHELXCD
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	44.590
High resolution limit [Å]	1.900
Number of reflections	52618
<I/σ(I)>	10.84
Completeness [%]	99.2
Redundancy	7.26

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	50 mM sodium acetate pH 4.6, 100 mM CaCl2 and 16% (v/v) 2-propanol