5N6U
Crystal structure of Beta-D-Mannosidase from Dictyoglomus thermophilum.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 200 |
| Detector technology | PIXEL |
| Collection date | 2015-03-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 139.250, 75.560, 217.880 |
| Unit cell angles | 90.00, 92.52, 90.00 |
Refinement procedure
| Resolution | 49.500 - 3.080 |
| R-factor | 0.20637 |
| Rwork | 0.204 |
| R-free | 0.25483 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2je8 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.771 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.500 | 3.140 |
| High resolution limit [Å] | 3.080 | 3.080 |
| Rmerge | 0.185 | 1.762 |
| Rmeas | 0.216 | 2.083 |
| Rpim | 0.110 | 1.093 |
| Number of reflections | 71693 | |
| <I/σ(I)> | 7.3 | |
| Completeness [%] | 98.9 | |
| Redundancy | 3.74 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 24% PEG400, 0.1 M Na-HEPES pH 7, 200 mM MgCl2 |
