5N6M
Structure of the membrane integral lipoprotein N-acyltransferase Lnt from P. aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-07-04 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.0003 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 152.730, 39.230, 102.250 |
Unit cell angles | 90.00, 116.26, 90.00 |
Refinement procedure
Resolution | 49.500 - 3.100 |
R-factor | 0.2295 |
Rwork | 0.225 |
R-free | 0.27290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5n6h |
RMSD bond length | 0.004 |
RMSD bond angle | 0.855 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | BUSTER |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 49.500 |
High resolution limit [Å] | 3.100 |
Number of reflections | 10159 |
<I/σ(I)> | 6.48 |
Completeness [%] | 98.9 |
Redundancy | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | 100 mM NaCitrate pH 5.0 30 % (v/v) PEG-500 DME 100 mM sodium acetate |