5N6M
Structure of the membrane integral lipoprotein N-acyltransferase Lnt from P. aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-04 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0003 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 152.730, 39.230, 102.250 |
| Unit cell angles | 90.00, 116.26, 90.00 |
Refinement procedure
| Resolution | 49.500 - 3.100 |
| R-factor | 0.2295 |
| Rwork | 0.225 |
| R-free | 0.27290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5n6h |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.855 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | BUSTER |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 49.500 |
| High resolution limit [Å] | 3.100 |
| Number of reflections | 10159 |
| <I/σ(I)> | 6.48 |
| Completeness [%] | 98.9 |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 100 mM NaCitrate pH 5.0 30 % (v/v) PEG-500 DME 100 mM sodium acetate |
