5N6H
Structure of the membrane integral lipoprotein N-acyltransferase Lnt from E. coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-09-07 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.99997 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.200, 142.300, 199.920 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.099 - 2.900 |
R-factor | 0.2204 |
Rwork | 0.219 |
R-free | 0.24940 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.728 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | CRANK2 |
Refinement software | BUSTER |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 49.100 |
High resolution limit [Å] | 2.900 |
Number of reflections | 693957 |
<I/σ(I)> | 9.5 |
Completeness [%] | 100.0 |
Redundancy | 19.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | 8 % (v/v) MPD 0.1 MES pH 6.0 0.4 M ammonium citrate |