5N5Z
Cryo-EM structure of RNA polymerase I in complex with Rrn3 and Core Factor (Orientation II)
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 7.7 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
