5N4L

Rat ceruloplasmin trigonal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-06
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.284800
Spacegroup name	P 32 2 1
Unit cell lengths	128.230, 128.230, 407.660
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	14.990 - 3.200
R-factor	0.1967
Rwork	0.193
R-free	0.26480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4enz
RMSD bond length	0.016
RMSD bond angle	2.048
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	14.990	3.300
High resolution limit [Å]	3.200	3.200
Rmerge	0.370	2.900
Rpim	0.028	0.410
Number of reflections	65234
<I/σ(I)>	9.8	2.2
Completeness [%]	99.2	99.2
Redundancy	19.1
CC(1/2)	0.891

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	MPD, KFormate