5N4H

Crystal structure of the D109N mutant of the mouse alpha-Dystroglycan N-terminal region

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-10
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.000
Spacegroup name	H 3
Unit cell lengths	71.740, 71.740, 143.990
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.997 - 1.701
R-factor	0.1603
Rwork	0.159
R-free	0.18240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1u2c
RMSD bond length	0.013
RMSD bond angle	1.270
Data reduction software	XDS (VERSION OCT 15, 2015)
Data scaling software	XSCALE (VERSION OCT 15, 2015)
Phasing software	PHASER (2.7.2)
Refinement software	PHENIX ((1.11_2561))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.096	1.098
Rmeas	0.103	1.181
Number of reflections	30354	2776
<I/σ(I)>	11.96	1.96
Completeness [%]	99.7	97.9
Redundancy	7.8	7.5
CC(1/2)	0.997	0.833

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	0.7 M Citrate Buffer, pH 7.0 5.5 mg/mL protein in 25 mM Tris, 150 mM NaCl, pH 7.5