5N4F

Prolyl oligopeptidase B from Galerina marginata - apo protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-16
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 2
Unit cell lengths	90.890, 105.620, 86.960
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.000 - 2.400
R-factor	0.2176
Rwork	0.216
R-free	0.25374
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.171
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall
Low resolution limit [Å]	54.000
High resolution limit [Å]	2.400
Number of reflections	31438
<I/σ(I)>	14.5
Completeness [%]	98.6
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293.15	30% PEG4000 and 100 mM MES buffer, pH 6.5.