5N4A
Crystal structure of Chlamydomonas IFT80
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-14 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 82.778, 195.403, 117.455 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.925 - 1.790 |
| R-factor | 0.1754 |
| Rwork | 0.174 |
| R-free | 0.20710 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.056 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (dev_1647) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.000 | 1.900 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.095 | 1.400 |
| Number of reflections | 173465 | 27521 |
| <I/σ(I)> | 11.3 | 1 |
| Completeness [%] | 99.5 | 97.3 |
| Redundancy | 6.6 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | 50 mM MES pH 6.0 and 100 mM Na-oxalate, 18C cryo-protected by the addition of 30% glycerol |
