5N41

Archaeal DNA polymerase holoenzyme - SSO6202 at 1.35 Ang resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-12-10
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97949
Spacegroup name	P 32 2 1
Unit cell lengths	61.752, 61.752, 35.363
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.876 - 1.351
R-factor	0.1622
Rwork	0.160
R-free	0.19810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5n35
RMSD bond length	0.009
RMSD bond angle	0.954
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575-000))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.900	1.400
High resolution limit [Å]	1.350	1.350
Rmerge	0.046	0.339
Number of reflections	16515	1178
<I/σ(I)>	28.2	3.1
Completeness [%]	95.2	68.6
Redundancy	4.8	3
CC(1/2)		0.839

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	294.15	OD280=17.7 mg/ml in 20 mM HEPES pH 7.5, 0.3 M NaCl, 1 mM MgCl2 and 1 mM b-ME mixed with 0.1 M Potassium thiocyanate and 30% w/v Polyethylene glycol monomethyl ether 2000 (Hampton Index)