5N3U
The structure of the complex of CpcE and CpcF of phycocyanin lyase from Nostoc sp. PCC7120
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-08-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 74.600, 59.825, 110.634 |
| Unit cell angles | 90.00, 99.08, 90.00 |
Refinement procedure
| Resolution | 57.050 - 1.890 |
| R-factor | 0.18368 |
| Rwork | 0.182 |
| R-free | 0.21616 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | In-house structure |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.970 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (6.5.018) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.050 | 2.010 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.060 | |
| Number of reflections | 36870 | |
| <I/σ(I)> | 21.45 | 4.53 |
| Completeness [%] | 95.3 | 82.2 |
| Redundancy | 6.53 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M sodium acetate pH 4.8, 5 % (w/v) PEG 10000 |
