5N3Q
cAMP-dependent Protein Kinase A from Cricetulus griseus in complex with fragment like molecule 3-Aminobenzamide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.3 |
Synchrotron site | BESSY |
Beamline | 14.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-09-18 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.895 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.576, 72.013, 97.588 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.395 - 1.310 |
R-factor | 0.1358 |
Rwork | 0.135 |
R-free | 0.15530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4wih |
RMSD bond length | 0.009 |
RMSD bond angle | 1.050 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.390 |
High resolution limit [Å] | 1.310 | 1.310 |
Rmeas | 0.076 | 0.532 |
Number of reflections | 88063 | 14042 |
<I/σ(I)> | 17.42 | 5.05 |
Completeness [%] | 99.9 | 99.5 |
Redundancy | 6.52 | 6.43 |
CC(1/2) | 0.990 | 0.930 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 277 | MES-BIS-TRIS Mega8-Solution DTT EDTA LiCl Methanol |