5N2Q
MobM Relaxase Domain (MOBV; Mob_Pre) bound to 26nt pMV158 oriT DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.8726 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.576, 52.853, 56.035 |
| Unit cell angles | 90.00, 95.88, 90.00 |
Refinement procedure
| Resolution | 55.730 - 2.000 |
| R-factor | 0.16975 |
| Rwork | 0.166 |
| R-free | 0.23843 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.135 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 55.730 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 16179 |
| <I/σ(I)> | 10.8 |
| Completeness [%] | 98.6 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 4.6 | 277 | 100 mM NaAc, pH 4.6 and 10% PEG 6000 |
