5N2G
Structure of the E9 DNA polymerase from vaccinia virus in complex with manganese
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-02-14 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.0714 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 134.007, 134.007, 230.189 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.000 - 2.780 |
R-factor | 0.18548 |
Rwork | 0.183 |
R-free | 0.22721 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5n2e |
RMSD bond length | 0.011 |
RMSD bond angle | 1.553 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.000 | 2.890 |
High resolution limit [Å] | 2.780 | 2.790 |
Rmeas | 0.108 | 0.958 |
Number of reflections | 59597 | |
<I/σ(I)> | 11.6 | 1.8 |
Completeness [%] | 99.1 | 99.2 |
Redundancy | 3.4 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.25 | 293 | 9-11% PEG 3000, 20-25% glycerol, 100 mM MES-NaOH pH 6.25, 5 mM MnCl2 |