5N2E
Structure of the E9 DNA polymerase from vaccinia virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 133.415, 133.415, 230.525 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 115.810 - 2.740 |
| R-factor | 0.18849 |
| Rwork | 0.186 |
| R-free | 0.23561 |
| Structure solution method | MIR |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.855 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.000 | 2.810 |
| High resolution limit [Å] | 2.740 | 2.740 |
| Rmeas | 0.077 | 0.728 |
| Number of reflections | 63014 | |
| <I/σ(I)> | 18.2 | 2.8 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5.3 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.25 | 293 | 9-11% PEG 3000, 20-25% glycerol, 100 mM MES-NaOH pH 6.25 |
