5N1U
Structure of xEco2 acetyltransferase domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2016-06-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.475, 66.287, 109.763 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.834 - 2.976 |
| R-factor | 0.2279 |
| Rwork | 0.223 |
| R-free | 0.26760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mxe |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.688 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.834 | 3.080 |
| High resolution limit [Å] | 2.976 | 2.980 |
| Rmerge | 0.297 | 1.160 |
| Number of reflections | 9148 | |
| <I/σ(I)> | 7.7 | 1.52 |
| Completeness [%] | 99.0 | |
| Redundancy | 6.1 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.2 M NaCl, 0.1 M imidazole pH 7 and 0.864 M ammonium phosphate |
