5N1A

Crystal structure of Utp4 from Chaetomium thermophilum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-23
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.8726
Spacegroup name	C 1 2 1
Unit cell lengths	203.027, 81.593, 112.300
Unit cell angles	90.00, 110.55, 90.00

Refinement procedure

Resolution	47.528 - 2.150
R-factor	0.1852
Rwork	0.183
R-free	0.22040
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	1.016
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall
Low resolution limit [Å]	47.530
High resolution limit [Å]	2.150
Number of reflections	93437
<I/σ(I)>	13.7
Completeness [%]	99.9
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	5% (w/v) 2-methyl-1,5-pentane-diol (MPD) 100 mM Tris-HCl pH 85