5N0S
Crystal structure of OphA-DeltaC6 mutant Y98A in complex with SAM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-10-14 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 163.906, 92.521, 85.332 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 85.330 - 1.950 |
| R-factor | 0.20663 |
| Rwork | 0.205 |
| R-free | 0.23335 |
| Starting model (for MR) | 5n0o |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.439 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.330 | 2.090 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.098 | 1.017 |
| Number of reflections | 93739 | |
| <I/σ(I)> | 6.7 | 1 |
| Completeness [%] | 98.4 | 95.1 |
| Redundancy | 5.9 | 4.5 |
| CC(1/2) | 0.996 | 0.657 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.3-0.4 M KSCN, 1.5-1.9 M sodium malonate and 0.1 M bicine pH 9.0 |
