5MZU

Crystal structure of the myosin chaperone UNC-45 from C. elegans (alternative conformation)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 61 2 2
Unit cell lengths	86.149, 86.149, 716.465
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.400 - 3.800
R-factor	0.2986
Rwork	0.298
R-free	0.31890
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.837
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (dev_2645)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.870
High resolution limit [Å]	3.800	10.290	3.800
Rmerge	0.085	0.034	0.367
Total number of observations	70085
Number of reflections	16050
<I/σ(I)>	7.4
Completeness [%]	93.5	59	97.9
Redundancy	4.4	3.2	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	292	0.1M Hepes pH 7.0, 10% PEG8000, 12% ethylene glycol