5MZ2
Rubisco from Thalassiosira antarctica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-24 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 118.300, 220.100, 124.351 |
| Unit cell angles | 90.00, 118.39, 90.00 |
Refinement procedure
| Resolution | 62.091 - 1.900 |
| R-factor | 0.1443 |
| Rwork | 0.143 |
| R-free | 0.17550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.922 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 98.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.117 | 0.388 |
| Number of reflections | 437345 | 43600 |
| <I/σ(I)> | 4.1 | |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 26% PEG 4000, 0.1M HEPES pH 8.5, 0.1M sodium chloride, 0.005M magnesium chloride, 0.01 sodium hydrogen carbonate |
