5MYF
Convergent evolution involving dimeric and trimeric dUTPases in signalling.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-01 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0721 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 43.642, 51.942, 148.418 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.026 - 1.850 |
| R-factor | 0.1797 |
| Rwork | 0.177 |
| R-free | 0.21860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5myd |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.521 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.030 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.024 | 0.350 |
| Number of reflections | 29551 | 4063 |
| <I/σ(I)> | 18.7 | |
| Completeness [%] | 99.5 | 96.5 |
| Redundancy | 11 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 296 | 28% PEG 6000, 0.5M LiCl2 y 0.1M Tris-HCl pH 8.5 |
