5MYD
Convergent evolution involving dimeric and trimeric dUTPases in signalling.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97942 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.890, 81.440, 81.530 |
| Unit cell angles | 90.00, 106.92, 90.00 |
Refinement procedure
| Resolution | 78.120 - 2.300 |
| R-factor | 0.22589 |
| Rwork | 0.224 |
| R-free | 0.26533 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.358 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 81.440 | 3.160 | 2.210 |
| High resolution limit [Å] | 2.100 | 3.000 | 2.100 |
| Rmerge | 0.048 | 0.450 | 0.350 |
| Number of reflections | 50736 | 7325 | |
| <I/σ(I)> | 8.4 | 2.1 | |
| Completeness [%] | 99.2 | 99.9 | 98.9 |
| Redundancy | 4 | 7.2 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 296 | 8% PEG 3350, 0.1 M Na-HEPES pH 7.5; 0.2M NaCl, 1.2 M ammonium sulphate and 0.03 M ammonium acetate |
| 2 |
