5MYC
Crystal structure of human 14-3-3 sigma in complex with LRRK2 peptide pS910
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-26 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.978620 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 82.517, 112.411, 62.689 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.848 - 1.459 |
R-factor | 0.1671 |
Rwork | 0.166 |
R-free | 0.18870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5btv |
RMSD bond length | 0.006 |
RMSD bond angle | 0.750 |
Data reduction software | XDS |
Data scaling software | XDS |
Refinement software | PHENIX ((1.11_2567: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.618 | 1.540 |
High resolution limit [Å] | 1.088 | 1.460 |
Rmerge | 0.124 | 1.086 |
Rmeas | 0.129 | |
Rpim | 0.035 | |
Number of reflections | 116840 | |
<I/σ(I)> | 14.99 | 2.2 |
Completeness [%] | 99.7 | 98.7 |
Redundancy | 4.6 | 11.8 |
CC(1/2) | 0.750 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 277 | HEPES, NaCl2, PEG400, Glycerol |