5MY2

KS-MAT DI-DOMAIN OF MOUSE FAS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-02
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.966
Spacegroup name	C 2 2 2
Unit cell lengths	216.565, 345.810, 145.229
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	183.540 - 2.700
R-factor	0.2189
Rwork	0.217
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.653
Data reduction software	iMOSFLM
Data scaling software	Aimless (0.5.1)
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	183.540	43.750	2.750
High resolution limit [Å]	2.700	14.790	2.700
Rmerge	0.154	0.046	0.912
Rmeas	0.177	0.052	1.048
Rpim	0.085	0.023	0.502
Number of reflections	147769
<I/σ(I)>	7.7
Completeness [%]	99.3	95.1	99.5
Redundancy	4.4	4.8	4.4
CC(1/2)	0.983	0.997	0.554

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	25 % w/v PEG 3350 0.2M Ammonium acteate 0.1M HEPES pH 7.5