5MXW

Crystal structure of yellow lupin LLPR-10.2B protein in complex with melatonin and trans-zeatin.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-2
Synchrotron site	MAX II
Beamline	I911-2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-15
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0000000
Spacegroup name	P 65
Unit cell lengths	74.400, 74.400, 67.310
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.590 - 1.570
R-factor	0.1858
Rwork	0.185
R-free	0.20590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qim
RMSD bond length	0.019
RMSD bond angle	1.866
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.590	46.590	1.660
High resolution limit [Å]	1.570	4.690	1.570
Rmerge	0.037	0.022	0.744
Rmeas	0.041	0.025	0.834
Total number of observations	147160
Number of reflections	29524	1132	4688
<I/σ(I)>	22.2	63.86	2.01
Completeness [%]	99.7	99	98.4
Redundancy	4.984	4.666	4.892
CC(1/2)	0.999	0.999	0.789

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3	292	1.4 M sodium citrate pH 6.3